• Users Online: 94
  • Print this page
  • Email this page


 
 Table of Contents  
ORIGINAL ARTICLE
Year : 2018  |  Volume : 2  |  Issue : 4  |  Page : 300-305

Prevalence of rifampicin-resistant pediatric tuberculosis by cartridge-based nucleic acid amplification test at the intermediate reference laboratory under revised national tuberculosis control program India: A multidimensional approach


State TB Demonstration Cum Training Centre, Intermediate Reference Laboratory, Kolkata, West Bengal, India

Date of Submission17-Aug-2018
Date of Decision15-Sep-2018
Date of Acceptance22-Sep-2018
Date of Web Publication11-Dec-2018

Correspondence Address:
Dr. Prasanta Kumar Das
State TB Demonstration Cum Training Centre, Intermediate Reference Laboratory, Kolkata - 700 010, West Bengal
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/bbrj.bbrj_114_18

Rights and Permissions
  Abstract 


Background: Tuberculosis (TB) still remains the major public health threat in India. Early diagnosis, so as to initiate early treatment is a priority as any delay, may complicate the prognosis further leading to the failure of an effective control. India accounts for 6% incidence of pediatric TB cases in a population that has 40% as estimated latent TB cases. Implementation of cartridge-based nucleic acid amplification tests (CBNAATs) in diagnosis of pediatric TB has augmented the detection rates. The study involves a retrospective multidimensional analysis of the increased pediatric TB case detection by CBNAAT at the Intermediate Reference laboratory, State TB Demonstration and Training Centre in West Bengal, India. A total of 14,088 samples from pediatric patients coming from all the districts were tested from May 2014 to April 2018. This included pulmonary (sputum, bronchoalveolar lavage, gastric lavage, and gastric aspirate) and extrapulmonary (cerebrospinal fluid [CSF], pus, ascitic fluid, pericardial fluid, lymph node aspirate) samples. Although detection levels showed variation in the nature of the samples, the study explored percentage contribution of the types of samples and the proportion positive rates among them. Percentage-wise propensity with regard to age, clinical presentations, type of samples, and smear result were observed. The predominant geographical location in terms of incidences and periodic prevalence were studied. The resistant cases were retested with line probe assay by MTBDRplus and V2 Hains for concordance analysis. This was done based on the parameters in the erstwhile evaluations. Methods: Pulmonary and extrapulmonary pediatric samples were tested on CBNAAT by Xpert Mycobacterium tuberculosis rifampicin (RIF) (Cepheid) based on the manufacturer's instruction. All necessary aseptic measures were taken. The data were captured in the Xpert software automatically during the tests and exported to the Microsoft Excel sheets for further analysis. A defined study design against each and every objective was set up. Results: It was found that a point prevalence of 6%–7% of pediatric TB exists among the tested specimens every quarter. The periodic prevalence was found to be 5%, the incidence rates ranged from 4.5% to 5%. RIF resistance detection showing a seasonal variation ranged from 13% to 15% among the CBNAAT-positive cases. Gastric lavage showed a major detection in children below 6 months of age of whom collecting sputum samples were difficult. This prompted suboptimal detection levels due to the dearth of sample collection modalities at the peripheries. CSF accounted for 2.37% of positivity. Conclusions: The study concluded that more skilled collection centers for biological specimens are required to address the undetected pulmonary TB cases among the pediatric age group, especially below the 6 months of age. About 5% prevalence and around 4.9% incidence is an alarming situation in the TB control scenario of West Bengal. Focus on the universal drug-susceptibility testing is a prerequisite.

Keywords: Cartridge-based nucleic acid amplification tests, Mycobacterium tuberculosis complex, pediatric tuberculosis, prevalence, Revised National Tuberculosis Control program, Xpert Mycobacterium tuberculosis rifampicin


How to cite this article:
Das PK, Ganguly SB, Mandal B, Khan A. Prevalence of rifampicin-resistant pediatric tuberculosis by cartridge-based nucleic acid amplification test at the intermediate reference laboratory under revised national tuberculosis control program India: A multidimensional approach. Biomed Biotechnol Res J 2018;2:300-5

How to cite this URL:
Das PK, Ganguly SB, Mandal B, Khan A. Prevalence of rifampicin-resistant pediatric tuberculosis by cartridge-based nucleic acid amplification test at the intermediate reference laboratory under revised national tuberculosis control program India: A multidimensional approach. Biomed Biotechnol Res J [serial online] 2018 [cited 2019 Aug 21];2:300-5. Available from: http://www.bmbtrj.org/text.asp?2018/2/4/300/247237




  Introduction Top


It is estimated that India accounts for 25% of global tuberculosis (TB) burden of the world. The pediatric population accounted for 6% of the total burden.[1] In West Bengal, India, before the availability of cartridge-based nucleic acid amplification test (CBNAAT), the pediatric TB was seen in 3.8% cases among the total population tested.[2]

As the initial diagnosis was solely dependent on smear microscopy, paucibacillary samples were left undetected. It is estimated that India accounts for around 40% of the latent TB infection.[3] The pediatric case detections were less owing to a dearth of a sensitive technology not being able to detect the underlying cause.[4],[5],[6] The drug resistance cannot be detected by smear microscopy. Nucleic acid amplification methods are able to detect Mycobacterium tuberculosis complex (MTBC) categorically along with the point mutations at the rifampicin (RIF) resistance determining region.[7] Moreover the analytical sensitivity of smear microscopy is not sufficient enough as it accounts for a poor-positive predictive value owing to its nonspecific detections.[8]

The following study explored the increased case detection for the pediatric group among variety of samples after the availability of CBNAAT in the remotest districts of West Bengal. Multidimensional analysis of the increased pediatric TB case detection by CBNAATs was undertaken.

Variation in percentages propensity with regard to age, clinical presentations, type of samples, and smear result were looked for. The predominant geographical location in terms of incidences and periodic prevalence were studied. The study also undertook concordance analysis of the RIF-resistant results with line probe assay (LPA).


  Methods Top


The study explores a multidimensional analysis of the CBNAAT performed on the samples among the pediatric population covering the entire state of West Bengal.

The objectives included the most common referral sites sending samples to Intermediate Reference Laboratory, Kolkata, the positivity and RIF resistance status among the referral sites. Detection rates with varying age among the pediatric groups and type of the sample (pulmonary as well as extrapulmonary). Smear results and its correlation to CBNAAT detection among the target groups. Concordance studies of the resistant cases with LPA.

Study duration

The retrospective study was undertaken covering a period from May 2014 to May 2018.

Sample selection

All the pediatric samples, pulmonary and extrapulmonary, irrespective of their geographical location that came to the Intermediate Reference Laboratory with a request for CBNAAT have been included in the study.

Study Design

The methods involve retrospective multidimensional analysis of the CBNAATs data of the pediatric samples coming to Intermediate Reference Laboratory, Kolkata, from May 2014 to May 2017. The proposal was reviewed and approved by Research and Ethical committee Intermediate Reference Laboratory, Kolkata India. The Ethics Committee Approval number STDC/IRL – CBNAAT Peadiatrc project no 012/ 2014 dated 5th Jan 2014 -2017.

Data were captured for the entire period from the recording and registration register, the study designed was worked out based on the available data using computational software.

Selective screening helped in tracking the contribution of the referral sites. The age and sample-related propensity in the case detection were also studied. The probe comprised not only the detection of MTBC by CBNAAT but also incidence and concordance studies of the RIF-resistant cases.

Before the processing of the sample for Gene Xpert around 20 μl was taken for making smears on grease-free slides. These slides were stained and examined under a fluorescent microscope.

The samples were processed as per the standard operating procedures led down by the GeneXpert MTB/RIF guidelines. For Xpert, around 2–5 ml of sample was taken. In case of cerebrospinal fluid (CSF), the test was run with at least of 1 ml of sample. The samples were mixed with double amount of buffered solution. The sample along with the buffer solution was made to stand for 15 min with intermittent gentle shaking in between for effective mixing. The tests were then run on CBNAAT machines as per the manufacturer's user manual.[9]

A 100μl of the buffer sample deposits (after centrifugation at 3000 g for 20 min) sent for LPA (Genotype MTBDRplus, V2 of Hains life sciences to see the concordance).[10]

Statistical analysis

Gastric lavage showed a major detection in children after sputum as a biological specimen contributing around 8.35% of the total positive detection. The contribution to RIF resistance was 11.1% as seen in other studies.[11] Data were captured on Microsoft Excel worksheets and age and sample-wise propensity results were expressed in terms of percentage. A Student's t-test was done between the paired data of the quarterly point, the prevalence showing P < 0.05 was obtained. Odds ratio between the smear results and CBNAAT positivity was calculated out using MedCalc Software (https://www.medcalc.org/calc/odds_ratio.php).[12]


  Results Top


It was observed that after sputum, gastric lavage contributed mostly in the pediatric case detection.

Their contribution toward RIF resistance varied from 12% to 16% among the positives CSF contributed to 3% of the pediatric samples. The MTB detection percentage and RIF resistance percentage ranged from 8% to 9% among the positives [Chart 1].



Prevalence and its significance

A Student's t-test was done between the quarterly point prevalence showing significance at 95% confidence interval (CI) (P < 0.05), and the point prevalence of MTB detection in the pediatric age group varied from 6% to 7% quarter-to-quarter period, a prevalence of MTB detection was 5%.

Point and periodic prevalence of RIF-resistant MTB among the population tested varied from 0.2% to 0.5%. Point prevalence of RIF resistance MTB among the detected found to range between 5% and 15% with a seasonal predominance during winter months.

Proportion positivity

With the sample size of 14,088, the MTB detected positive by CBNAAT was 979 (6.9%). Proportion of positive results was found to be 0.069 with the lower bound of 0.065 and the upper bound of 0.071 at 95% CI.

The proportion of RIF resistance among the positives ranged from 0.110 to 0.153 at 95% CI.

Sample- and age-wise detection

Among the sample types, the gastric lavage contributed the most after sputum. The proportion positivity varied from one sample to other. As different samples came in different numbers, it was not equitable to comment on the percentage positivity. Similar number of different samples would have helped in achieving significant data [Table 1].
Table 1: Percentage detection in different age groups

Click here to view


Detection was poor in all most all the biological specimens in the age group below 6 months, however, the gastric aspirate and gastric lavage helped in significant case detection levels after sputum.

The percentage detection was maximum in the age group between 1 and 5 years, and >1 year (29.51% and 57.27%, respectively), followed by the age group <1 year (10.42%) [Table 2].
Table 2: The age-wise contribution toward positive case detection by cartridge-based nucleic acid amplification tests

Click here to view


The overall percentage detection was minimum in the age group below 6 months (2.78%).

Gastric Lavage contributed to 8.35% of the total positive detection.

CSF contributed to almost 2.37% of the entire positives detected, with around 13.79% RIF resistance among the CBNAAT-CSF positives.

Geographical distribution

The MTB cases detected in the urban slum area of overcrowded, the urban slum area and sporadic cases of multidrug-resistant (MDR) among them is an alarming threat to the community.

Smear microscopy and cartridge-based nucleic acid amplification test positivity

On calculating odds ratio between the smear results and CBNAAT positivity, it was found an odds ratio of >1 with CI 95% with P < 0.0001 shows higher detection[12] of MTB through CBNAAT in smear-positive cases than negative cases; however, the analytical sensitivity of CBNAAT and smear microscopy is 100 CFU/ml[13] and 5000–10000 colony-forming unit (CFU)/ml[14] respectively.

Based on the higher sensitivity and high-positive predictive value of CBNAAT, the positivity among the smear negative showed a substantial percentage. Forty-eight percent though the study showed higher detection among the smear negatives.[15]


  Discussion Top


Among the top ten causes of death worldwide, TB accounts for one of the most important factors; approximately, 1.2 million children became ill and 0.17 million died with it.[16] Five-hundred children deaths due to TB and over three quarters of a million children fall ill with TB each year.[17] Clinical diagnosis being nonspecific and considerable interreader variation in radiological findings[18] prompts for a diagnostic technology of a high-positive predictive value. Microbiological confirmations would help in giving a clearer picture for diagnosis and prognosis of disease in the course of treatment.[19]

The Intermediate Reference laboratory at Kolkata under State TB demonstration and training center (Government of West Bengal, India) has a molecular biology setup that runs LPA for antitubercular drugs as well as CBNAATs. The laboratory is well managed with internal quality control (QC) and external QC done by the National Reference Laboratories on behalf of Central TB Division, Government of India.

A total of 14,088 numbers of pediatric specimens were tested in a span of 4 years (2014–2018) coming from all the districts of West Bengal, India.

The specimens comprised several samples that included pulmonary as well as extrapulmonary samples. Pulmonary samples comprised sputum, induced sputum, and bronchoalveolar lavage and extrapulmonary samples comprised gastric lavage, ascitic fluid, plural fluid, pericardial fluid, lymph nodes (axillary and cervical), CSF, synovial fluids, and tissues.

The study undertook multidimensional cohort analysis of the increased case detection rates with regard to the referrals, nature of the samples, and age group contribution to the positivity (whether indicative of any age-wise pattern). The retrieval rates in paucibacillary samples and the occurrence of RIF-resistant MTBC strains among these samples were looked for. The strains which were found resistant by CBNAAT were subjected to LPA by MTBDRplus and V2 Hain for concordance analysis. Clinical manifestation correlating to an affirmative detection was also a component of the study smear-related correlation and their outcomes were analyzed.

Sample- and age-wise positivity

Gastric lavage showed a maximum detection after sputum. It is suggested for pediatric population for <1 year of age collection of other biological specimens such as gastric aspirate, gastric lavage, and induced sputum increases the yield where sputum collection is difficult.

Among the age group between 6 months and 1 year, the gastric lavage showed a detection rate of almost 16% after sputum which showed an MTB detection rate of 46.6%. Detection was mostly found in the age group between 6 months and 1 year.

Although most of the samples that frequented were sputum, CSF from inpatient formed a major portion (8.6%) of the total samples tested. Detection was high in CSF among the age group above 6 months. The percentage of RIF-resistant strain detected within this group varied from 8% to 7%.

Geographical location

The study also tried to find out the possibility of predominance of pediatric TB in a particular specific geographical location.

The MTB cases detected in the urban slum area of overcrowded, the urban slum area and sporadic cases of MDR among them is an alarming threat to the community.

Most of the pediatric TB cases were referred from the pediatric hospitals. TB units, peripheral health institutions, and medical colleges together contributed only 10%–12% of the total intake. The majority of the samples came from the government pediatric hospital. A substantial number of CSF (8.68%) came from In patients Department (IPD) of these hospitals.

Private practice involvement was found to be significant. Gastric lavage and gastric aspirate from the pediatric hospital augmented the detection rates. The scope of widening the pediatric TB care support by increasing the number of skilled collection center across the state will help in further increase in the detection rates.

However, the MDR detection cases during the study duration were dispersed throughout and not confined to the urban slum area.

Smear and cartridge-based nucleic acid amplification test positivity

The odds ratio corroborates with the higher analytical sensitivity of CBNAAT. The study showed CBNAAT was able to capture 48% of smear-negative samples.

Prevalence and its significance

The correlation between the quarterly prevalence rates was found to be significant using Student's t-test.

At 95% CI (P < 0.05), point prevalence of MTB detection in the pediatric age group varied from 3% to 5% quarter-to-quarter period, a prevalence of MTB detection was found to be 3.88%.

Point and periodic prevalence of RIF-resistant MTB among the population tested varied from 0.2% to 0.5%. Point prevalence of RIF resistance MTB among the detected found to vary between 5% and 15% with a seasonal predominance during summer months. This corroborated with the earlier findings.

Five percent prevalence and around 4.9% incidence is an alarming situation in the TB control scenario of West Bengal. This statistics is an alarming threat to the community and a rationality to be kept for implementation of universal drug susceptibility testing.

Proportion positivity

With the sample size of 14,088, the MTB detected positive by CBNAAT was 537 (3.8%) [Table 3]. Proportion of positive results was found to be 0.038 with the lower bound of 0.035 and the upper bound of 0.041 at 95% CI.
Table 3: Sample type contribution toward Mycobacterium tuberculosis detection and rifampicin resistance detection

Click here to view


The proportion of RIF resistance among the positives ranged from 0.116 to 0.146 at 95% CI.

Clinical correlation showed cough and fever as the most correlated manifestations.

Cough predominated mainly on the higher age group.

Cartridge-based nucleic acid amplification test and line probe assay concordance

The confirmed RIF-resistant MTB through CBNAAT was subjected twice in different batches for LPA (MTBDRplus and V2 Hains) to further validate and establish the concordance with CBNAAT.

Ninety percent result showed concordance with Line Probe Assay. However, 5% of cases were sensitive to RIF through LPA and 5% were found negative for MTB on repeated test on LPA. The established discordance of two molecular techniques showed varied specificity and sensitivity.

It has been observed that sputum collection has been an ordeal for pediatric population showing symptoms of pulmonary TB. The study showed substantial percentage detection of pediatric TB in pulmonary samples other than sputum. The group consisted of bronchoalveolar lavage, bronchial brushings, tracheal aspirates, and induced sputum. It was found that gastric lavage formed the majority of the cases from which TB was detected after sputum. This was observed from the percentage positivity of the different samples.

The odds ratio shows chances of getting CBNAAT detection is not dependent of smear positivity, around 45.5percent of smear negative turned out to be CBNAAT positive. Although most of the samples carried either low or very low-bacillary load.[12] This corroborates with the analytical sensitivity studies on CBNAAT as compared to the smear microscopy. However, it was found in earlier studies, the percent of samples were nontuberculous mycobacterium (NTM) which became CBNAAT negative even after smear positivity. The extensive study did not show a single case being smear positive while CBNAAT negative.


  Conclusion Top


The study showed the paramount importance of samples other than sputum, in detecting pulmonary TB in the age group between 6 months and 1 year. Gastric lavage and gastric aspirate contributed to 15% and 16%, respectively, in the pediatric case detection rates. A substantial numbers when translated to absolute figures remain undiagnosed due to dearth of appropriate sample collection.

Point prevalence of 6%–7% pediatric TB is seen in West Bengal, India. More units for sample collection would be of great help augmenting the case detection further. Detection from the sputum was more in moderate age groups (between 6 months and 1 year), however, the gastric lavage contributed significantly in the age group below 6 months. Around 40% cases which were missed by Fluorescent Microscopy using standard national guidelines[20] were detected by Nucleic acid amplification tests.

The study show MTB detection 3% among the CSF in West Bengal which stands as a public health challenge. CBNAAT was able to capture several of smear-negative paucibacillary cases. Odds ratio >1 depicted that the chances of getting a CBNAAT positive among the smear-positive cases were >90%; however, the percent of NTM among the missed 10% could be done with additional resources.

Acknowledgments

We are thankful to all staff at Intermediate Reference Laboratory, West Bengal, India, for providing technical inputs and all patients suffering and the staffs contributing for their dedication.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
TB India 2017. Revised National Tuberculosis Control Programme. Annual Status Report. Available from: http://www.TBCINDIA. gov.in/WriteReadData/TB%20India%202017.pdf.; [Last accessed on 2018 Sep 06].  Back to cited text no. 1
    
2.
Mukherjee A, Chowdhury R, Singla R, Saha I, Dutta R, Das T, et al. Comparison between childhood and adult tuberculosis in a rural tuberculosis unit of West Bengal: A retrospective study. Lung India 2014;31:116-20.  Back to cited text no. 2
[PUBMED]  [Full text]  
3.
Mahmood T. 40% of India's Population Play Host to the TB Bacillus as a Latent TB; 2016. Available from: http://www.oneindia.com/feature/40-percent-of-india-s-population-play-host-the-tb-bacillus-as-latent-tuberculosis-2049544.html.  Back to cited text no. 3
    
4.
Raj A, Singh N, Mehta PK. Gene Xpert MTB/RIF assay: A new hope for extra-pulmonary tuberculosis, 2014. J Pharm 2012;2:83-9. Available from: http://www.iosrphr.org/papers/v2i1/N021083089.pdf. [Last accessed on 2014 Aug 25].  Back to cited text no. 4
    
5.
Chakravorty S, Sen MK, Tyagi JS. Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology. J Clin Microbiol 2005;43:4357-62.  Back to cited text no. 5
    
6.
Raizada N, Sachdeva KS, Swaminathan S, Kulsange S, Khaparde SD, Nair SA, et al. Piloting upfront Xpert MTB/RIF testing on various specimens under programmatic conditions for diagnosis of TB and DR-TB in paediatric population. PLoS One 2015;10:e0140375.  Back to cited text no. 6
    
7.
Rufai SB, Kumar P, Singh A, Prajapati S, Balooni V, Singh S, et al. Comparison of Xpert MTB/RIF with line probe assay for detection of rifampin-monoresistant Mycobacterium tuberculosis. J Clin Microbiol 2014;52:1846-52.  Back to cited text no. 7
    
8.
Desikan P. Sputum smear microscopy in tuberculosis: Is it still relevant? Indian J Med Res 2013;137:442-4.  Back to cited text no. 8
[PUBMED]  [Full text]  
9.
XPERT MTB/RIF User's Manual. Available from: http://www.///C:/Users/ USER/Downloads/MTBDRplusV2_0615_304A 06 02%20 (1).pdf. [Last accessed on 2018 Oct 27].  Back to cited text no. 9
    
10.
Genotype MTBDRplusV2file. Available from: https://www.ghdonline.org/uploads/MTBDRplusV2_0212_304A-02-02.pdf. [Last accessed on 2018 Oct 27]  Back to cited text no. 10
    
11.
Iravane JA, fatema SA, Damle Ajit S. Genexpert for gastric lavage to diagnose pulmonary tuberculosis in children international J Microbiol Res 2017;9:1-4.  Back to cited text no. 11
    
12.
Medical Software. American Society for Microbiology; 2010. Available from: https://www.medcalc.org/calc/odds_ratio.phCopyright©. All Rights Reserved. [Last accessed on 2018 Oct 27].  Back to cited text no. 12
    
13.
Helb D, Jones M, Story E, Boehme C, Wallace E, Ho K, et al. Rapid detection of mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J Clin Microbiol 2010;48:229-37.  Back to cited text no. 13
    
14.
Bodmer T, Ströhle A. Diagnosing pulmonary tuberculosis with the Xpert MTB/RIF test. J Vis Exp 2012;62:e3547.  Back to cited text no. 14
    
15.
Lombardi G, Di Gregori V, Girometti N, Tadolini M, Bisognin F, Dal Monte P, et al. Diagnosis of smear-negative tuberculosis is greatly improved by Xpert MTB/RIF. PLoS One 2017;12:e0176186.  Back to cited text no. 15
    
16.
World Health Organization. Global Tuberculosis Report. World Health Organization; 2016.  Back to cited text no. 16
    
17.
World Health Organization. Roadmap for Childhood Tuberculosis: Towards Zero Deaths. Geneva: World Health Organization; 2013.  Back to cited text no. 17
    
18.
Hesseling AC, Schaaf HS, Gie RP, Starke JR, Beyers N. A critical review of diagnostic approaches used in the diagnosis of childhood tuberculosis. Int J Tuberc Lung Dis 2002;6:1038-45.  Back to cited text no. 18
    
19.
Zar HJ, Hanslo D, Apolles P, Swingler G, Hussey G. Induced sputum versus gastric lavage for microbiological confirmation of pulmonary tuberculosis in infants and young children: A prospective study. Lancet 2005;365:130-4.  Back to cited text no. 19
    
20.
Available from: http://www.stoptb.org/wg/gli/assets/documents/tb%20microscopy%20handbook_final.pdf. [Last accessed on 2018 Oct 27].  Back to cited text no. 20
    



 
 
    Tables

  [Table 1], [Table 2], [Table 3]



 

Top
 
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
Abstract
Introduction
Methods
Results
Discussion
Conclusion
References
Article Tables

 Article Access Statistics
    Viewed574    
    Printed61    
    Emailed0    
    PDF Downloaded80    
    Comments [Add]    

Recommend this journal


[TAG2]
[TAG3]
[TAG4]