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ORIGINAL ARTICLE
Year : 2019  |  Volume : 3  |  Issue : 4  |  Page : 228-232

Screening and optimization of staphylokinase from Staphylococcus aureus isolated from nasal swab of healthy students in Himachal Pradesh University, India


1 Department of Microbiology, Maharishi Markandeshwar Medical College and Hospital, Solan, Himachal Pradesh, India
2 Department of Biotechnology, Himachal Pradesh University, Shimla, Himachal Pradesh, India

Correspondence Address:
Dr. Sameer Singh Faujdar
Department of Microbiology, Maharishi Markandeshwar Medical College and Hospital, Kumarhatti, Solan - 173 229, Himachal Pradesh
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/bbrj.bbrj_128_19

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Background: One of virulence factors produced by Staphylococcus aureus is staphylokinase (SAK), which enhances their proteolytic activity leading to tissue damage and improving bacterial invasiveness. In the present study, we estimated the ability to produce SAK by S. aureus isolates from nasal carriers. We would like to verify relationship between SAK production and different S. aureus isolates. Methods: In this study, all nasal swab samples from healthy nasal carriers were collected and further processed in the Department of Biotechnology, Himachal Pradesh University. All S. aureus isolates were screened for SAK and optimization was done. Results: Out of all S. aureus isolates 20% isolates were positive for SAK production. Isolate SAK-24 shown increase in SAK production after optimization and response surface methodology (RSM). Conclusion: Production of SAK varies from strain to strains. SAK production can be increased by optimization and RSM.


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