Biomedical and Biotechnology Research Journal (BBRJ)

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 2  |  Issue : 1  |  Page : 46--52

Comparative study of LoopampTM Mycobacterium tuberculosis Complex Kit for Rapid Detection of Mycobacterium tuberculosis complex in cameroon


Valerie Flore Donkeng Donfack1, Laure Ngando2, Eric Walter Yone Pefura3, Dieudonné Shubesi Che1, Ghislaine Ateba3, Jean Joel Rim Bigna4, Jean Louis Abena Foe5, Christopher Kuaban6, Sara Eyangoh1 
1 Tuberculosis National Reference Laboratory, Centre Pasteur of Cameroon, International Pasteur Institutions Network, Yaoundé, Cameroon
2 Faculty of Medicine and Biomedical Sciences, University of Yaoundé, Yaoundé, Cameroon
3 Jamot Hospital, Tuberculosis Diagnostic and Treatment Center, Yaoundé, Cameroon
4 Epidemiology Unit, Centre Pasteur of Cameroon, Yaoundé, Cameroon
5 National Tuberculosis Program, Yaoundé, Cameroon
6 Faculty of Health Sciences, University of Bamenda, Bamenda, Cameroon

Correspondence Address:
Dr. Valerie Flore Donkeng Donfack
Tuberculosis National Reference Laboratory, Centre Pasteur of Cameroon, International Pasteur Institutions Network, P.O. 1274 Yaoundé
Cameroon

Background: The most practical test for identifying tuberculosis (TB) in developing countries remains smear microscopy. However, due to its low sensitivity, a new point-of-care diagnostic method has been developed. The purpose of this study was to assess the performance of TB-Loop-mediated isothermal amplification (TB-LAMP) test on sputum samples of suspected TB cases. Methods: Suspected pulmonary TB patients (527) from Jamot Hospital and without any history of anti-TB treatment were consecutively included in the study. Smear microscopy, TB-LAMP, GeneXpert® MTB/RIF, and liquid culture using BACTEC 960 Mycobacteria Growth Indicator Tube (MGIT) were performed on sputum samples collected from these patients. The sensitivity and specificity of TB-LAMP were compared with smear microscopy and GeneXpert® MTB/RIF. MGIT culture was the gold standard. Results: TB-LAMP and smear microscopy showed sensitivities of 82.6% (95% confidence interval [CI], 76.9–87.2) and 53.6% (95% CI, 46.8–60.3), respectively, and specificities of 96.0% (95% CI, 93.2–97.7) and 99.0% (95% CI, 97.1–99.7), respectively. The sensitivity and specificity of TB-LAMP were similar to GeneXpert®, (89.9%; 95% CI, 85.0–93.3 and 97.0%; 95% CI, 94.4–98.4). Conclusion: TB-LAMP is more sensitive than currently used microscopy. It presents a favorable diagnostic tool for TB in peripheral laboratories with limited equipment, such as those in developing countries.


How to cite this article:
Donfack VF, Ngando L, Pefura EW, Che DS, Ateba G, Bigna JJ, Abena Foe JL, Kuaban C, Eyangoh S. Comparative study of LoopampTM Mycobacterium tuberculosis Complex Kit for Rapid Detection of Mycobacterium tuberculosis complex in cameroon.Biomed Biotechnol Res J 2018;2:46-52


How to cite this URL:
Donfack VF, Ngando L, Pefura EW, Che DS, Ateba G, Bigna JJ, Abena Foe JL, Kuaban C, Eyangoh S. Comparative study of LoopampTM Mycobacterium tuberculosis Complex Kit for Rapid Detection of Mycobacterium tuberculosis complex in cameroon. Biomed Biotechnol Res J [serial online] 2018 [cited 2021 Dec 7 ];2:46-52
Available from: https://www.bmbtrj.org/article.asp?issn=2588-9834;year=2018;volume=2;issue=1;spage=46;epage=52;aulast=Donfack;type=0