Biomedical and Biotechnology Research Journal (BBRJ)

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 2  |  Issue : 1  |  Page : 53--58

Studies on 3H-levamisole binding to murine splenic lymphocytes, normal, malignant human lymphocytes and fate of levamisole in cell culture


B Nageshwari1, Ramchander Merugu2 
1 Department of Biotechnology, Government College Autonomous, Rajamahendravaram, Andhra Pradesh, India
2 Department of Biochemistry, Mahatma Gandhi University, Nalgonda, Telangana, India

Correspondence Address:
Dr. B Nageshwari
Department of Biotechnology, Government College Autonomous, Rajamahendravaram, Andhra Pradesh
India

Background: Levamisole at high concentrations has been shown to have anticancer and immunosuppressive actions. Methods: In the present study, fate of levamisole in cell culture and 3 H-levamisole binding to murine splenic lymphocytes, normal and malignant human lymphocytes has been investigated. Results: High-performance liquid chromatography analysis of cell culture supernatants of myeloma cells treated with levamisole has shown that products of levamisole appeared with progressive culture period indicating a metabolic transformation. 3 H-levamisole binding assays indicate that the binding was signifi cantly higher in lysates of lipopolysaccharide-stimulated murine splenic lymphocytes as compared to whole cells. Conclusion: The degradation of levamisole could be one more possible reason for the high concentration of levamisole required to get the desirable cytotoxic effect on myeloma cells.


How to cite this article:
Nageshwari B, Merugu R. Studies on 3H-levamisole binding to murine splenic lymphocytes, normal, malignant human lymphocytes and fate of levamisole in cell culture.Biomed Biotechnol Res J 2018;2:53-58


How to cite this URL:
Nageshwari B, Merugu R. Studies on 3H-levamisole binding to murine splenic lymphocytes, normal, malignant human lymphocytes and fate of levamisole in cell culture. Biomed Biotechnol Res J [serial online] 2018 [cited 2021 Nov 27 ];2:53-58
Available from: https://www.bmbtrj.org/article.asp?issn=2588-9834;year=2018;volume=2;issue=1;spage=53;epage=58;aulast=Nageshwari;type=0