Biomedical and Biotechnology Research Journal (BBRJ)

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 2  |  Issue : 1  |  Page : 63--67

A comparison of three diagnostic platforms for the detection of influenza A and B in children


Furqan Kabir1, Marvi Tariq2, Fatima Aziz1, Syed Imran Rizvi1, Shahida Qureshi1, Asad Ali1, Najeeha Talat Iqbal3 
1 Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan
2 Aga Khan Medical College, Aga Khan University, Karachi, Pakistan
3 Department of Paediatrics and Child Health; Department of Biological and Biomedical Sciences, Aga Khan University, Karachi, Pakistan

Correspondence Address:
Dr. Najeeha Talat Iqbal
Department of Paediatrics and Child Health and Biological and Biomedical Sciences, Aga Khan University, Stadium Road, P.O Box  3500, Karachi
Pakistan

Background: Viral flu is the predominant cause of hospitalization in young children, which invariably leads to enhanced morbidity and mortality in children in developing countries. Initial treatment of viral flu is based on presumptive diagnosis. Bedside testing is not common in clinical settings because of variable sensitivity and specificity of rapid tests in different settings. Methods: To address this issue, we evaluated the performance of Binax influenza A/B rapid testing kit against two robust molecular platforms (quantitative real-time polymerase chain reaction [qRT-PCR] and TaqMan array card [TAC]) in 24 nasopharyngeal (NP) swabs, collected from children under 5 years of age. Results: Binax was found to be less sensitive (56%), but 100% specific compared to qRT-PCR (100%) and TAC (>100%). Using TAC cards, 75% of samples were found to be coinfected with other bacterial and viral targets. Conclusion: Binax flu is suitable for bedside testing in clinical and community settings. The negative results of Binax should be interpreted with caution and confirmed by rapid molecular tests.


How to cite this article:
Kabir F, Tariq M, Aziz F, Rizvi SI, Qureshi S, Ali A, Iqbal NT. A comparison of three diagnostic platforms for the detection of influenza A and B in children.Biomed Biotechnol Res J 2018;2:63-67


How to cite this URL:
Kabir F, Tariq M, Aziz F, Rizvi SI, Qureshi S, Ali A, Iqbal NT. A comparison of three diagnostic platforms for the detection of influenza A and B in children. Biomed Biotechnol Res J [serial online] 2018 [cited 2021 Dec 7 ];2:63-67
Available from: https://www.bmbtrj.org/article.asp?issn=2588-9834;year=2018;volume=2;issue=1;spage=63;epage=67;aulast=Kabir;type=0