Biomedical and Biotechnology Research Journal (BBRJ)

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 5  |  Issue : 1  |  Page : 27--34

Computer-assisted multi-epitopes T-cell subunit Covid-19 vaccine design


Abdulwahid Abaukaka Yahaya1, Salihu Sanusi2, Fatima Umar Malo3 
1 Department of Medical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Toxicology and Pharmacology, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Chemistry, Nasarawa State University, Keffi, Nigeria

Correspondence Address:
Dr. Abdulwahid Abaukaka Yahaya
Department of Medical Biotechnology, Tehran University of Medical Sciences, Tehran
Iran

Background: The world is currently facing the coronavirus disease-2019 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Researchers from different parts of the world have employed diverse approaches to create a safe and effective vaccine as it saves millions of lives. Vaccines are created from the viral particle to train the body for a natural defense against invading pathogens. It is important to understand the concept of the vaccine design, especially the multi-epitope T-cells subunit vaccine. Methods: In this regard, we employed bioinformatics and immunoinformatic tools to illustrate the concept of the computer-based vaccine design. The computational methods consist of evaluation and selection of SARS-CoV-2 structural proteins, prediction of cytotoxic T-lymphocyte (CTL) epitopes, prediction of helper T-cell (HTL) epitope, multi-epitope vaccine candidate construct, antigenicity and allergenicity prediction of the designed candidate vaccine, physiochemical properties and solubility evaluation, secondary/tertiary structure prediction, refinement and validation of model vaccine tertiary structure, molecular docking of fusion proteins and Toll-like receptor 9 protein, and in silico cloning of the vaccine. Results: A total of 454 amino acid sequences were generated from CTL and HTL epitopes. The query solubility value (QuerySol) of the vaccine construct was 0.419, including the human β-defensin-2 adjuvant and peptide linkers. A circular clone of vaccine and pEX-C-His plasmid was achieved after in silico ligation using the annealed primer. Conclusion: Here, we provide essential information on computer-assisted multi-epitopes T-cell subunit vaccine design.


How to cite this article:
Yahaya AA, Sanusi S, Malo FU. Computer-assisted multi-epitopes T-cell subunit Covid-19 vaccine design.Biomed Biotechnol Res J 2021;5:27-34


How to cite this URL:
Yahaya AA, Sanusi S, Malo FU. Computer-assisted multi-epitopes T-cell subunit Covid-19 vaccine design. Biomed Biotechnol Res J [serial online] 2021 [cited 2021 Aug 5 ];5:27-34
Available from: https://www.bmbtrj.org/article.asp?issn=2588-9834;year=2021;volume=5;issue=1;spage=27;epage=34;aulast=Yahaya;type=0