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  Indian J Med Microbiol
 

Figure 8: Cloning of vaccine construct into the pEX-C-His plasmid. (a) Restriction analysis of the plasmid to identified key restriction enzymes. (b) Gel purification after restriction digestion. (c) Vaccine fragment designed from the multi-epitope design. (d) PCR on vaccine fragment to aneal primer, cozac sequence, and restriction enzymes. (e) Gel purification of the vaccine PCR product and (f) a circular clone of the vaccine into pEX-C-His plasmid

Figure 8: Cloning of vaccine construct into the pEX-C-His plasmid. (a) Restriction analysis of the plasmid to identified key restriction enzymes. (b) Gel purification after restriction digestion. (c) Vaccine fragment designed from the multi-epitope design. (d) PCR on vaccine fragment to aneal primer, cozac sequence, and restriction enzymes. (e) Gel purification of the vaccine PCR product and (f) a circular clone of the vaccine into pEX-C-His plasmid